Expressed gene
iCre, improved crerecombinase, bacteriophage P1
 

Site of expression
supraoptic nucleus, paraventricularnuclei, the magnocellularsubnuclei of PVN

A P2A-iCre sequence cassette was inserted upstream 3'UTR of the Oxt gene. This strain was maintained on a Sprague Dawley genetic background.

(A) Schematic of AAV-Retro-DIO-YFP viral injection in OXT-Cre rats.(B) Immunostaining of YFP with OXT-NP on viral injected OXT-Cre rat brain slices. Co-localization of YFP with OXT-NP is indicated with white triangles.(C) Parvocellular neurons in the PVN were negative for YFP.(J) Schematic injection and chemogenetic inhibition of Magno-OXT neurons with AAV-Retro-DIO-hM4Di.(K) Representative images showing the viral infection of Magno-OXT neurons. Co-localization of mCherry with OXT-NP is indicated with white triangles.(source: Zhang B et al.,2021)